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Therefore we conclude that the chosen spectral regime detects the same broad range of replicating microbes as the traditional visual plate counting method.
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These results suggest that WAD can perform well across a range of replicate numbers.
After preprocessing (normalization and derivative calculation), statistical tests were performed over the range of replicate spectra (600 4000 cm–1) by use of Unscrambler X software.
Once these values are entered the program calculates the relative and effective sample size of each DNA pool for a range of replicate array values, and provides a corresponding table of values as seen in Figure 7A and 7B.
In the second part, we used these estimates to calculate the effective sample size of a DNA pool given a range of replicate array values, and provide an online tool to allow readers to do the same.
We have prepared a simple online tool, PoolingPlanner (available at http://www.kchew.ca/PoolingPlanner/), which calculates the effective sample size (ESS) of a DNA pool given a range of replicate array values.
We also present a simple online tool, PoolingPlanner, which uses our empirical variance estimates as default values to calculate the effective sample size (ESS) of a DNA pool given a range of replicate array values (available at http://www.kchew.ca/PoolingPlanner/). PoolingPlanner also accepts user-supplied values for variance estimates.
Surprisingly, the compounds are inactive against a range of apicomplexans replicating in nucleated cells, including Toxoplasma gondii.
The model was extensively tested to ascertain that it is capable of replicating flux ranges and phenotypes that have been documented for the wild-type (WT) organism and its mutants.
It has been found that proposed MCL design should not only be capable of replicating a wide range of cardiac operating scenarios, but also the pumping system should better represent LV for simulating various cardiovascular diseases, particularly muscular dysfunctions.
The two-site model is capable of replicating the full range of effects incompatible with the single-site framework.
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