Exact(8)
The components that are brought together in this single structure endow it with an extraordinary range of receptor and signaling functions.
Notably, the FRET/receptor ratio was constant over a wide range of receptor densities, including expression levels similar to the endogenous levels in the brain (Maurel et al. 2008).
Mutant receptors with a variety of side-chain replacements still accessed both the ON and OFF signaling states, suggesting that core signaling complexes produce kinase activity over a range of receptor conformations and dynamic motions.
This range of receptor density is consistent with the density of GPCRs that form homo- and hetero-dimers on the membrane of different cell lines used in G-protein signaling experiments [38].
These studies were performed in a variety of cell lines, both normal and transformed, that exhibited a range of receptor expression levels; they were performed with two different ligands (EGF and TGFα); and they were performed in the presence and absence of serum to control for environmental factors.
The pathway is activated by a range of receptor tyrosine kinases.
The variance in ectopic expression of EGFR-mCitrine was thereby exploited to sample a broad range of receptor expression levels.
Furthermore, a linear biological response is observed only at doses that result in the lowest range of receptor occupancy (Daston et al. 2003; Welshons et al. 2003).
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