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A low flip angle was shown to reduce the apparent recovery rate of the IR-bSSFP time course, thus extending the dynamic range of quantification.
Despite being rapid and simple the method is thorough and provides a linear range of quantification of the drug from 10 nmol/ml to 1.4 μmol/ml.
A range of quantification between 10 nM and 100 μM was obtained, which shows the feasibility of detecting small molecules using aptamers with high sensitivity.
However, this response entails that the quantifier ∀x cannot be chosen to range over absolutely all sets; for if it could be so chosen, we would not be able deny that R is in this range of quantification.
The established Campylobacter real-time PCR assay showed a 7-log-wide linear dynamic range of quantification (R2 = 0.99) with a detection limit of 200 Colony Forming Units of Campylobacter per gram of feces.
Despite its limitations in the variety of target transcripts and in the dynamic range of quantification compared with RNA-Seq technology (Sultan et al. 2008; Wang et al. 2009), it is well established and is still a major platform for transcriptome analysis of sequenced organisms.
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A range of quantifications are made possible on CSSS using computer-assisted image analysis.
The reproducibility was determined on the total linear quantification range of both curves confirming the accuracy of the technical set-up in the complete ranges of quantification over time.
The data obtained from our assay confirmed the reliability and accuracy of the technical set-up over time and over the complete and the very low ranges of quantification.
To determine the linear calibration range, limit of quantification and limit of detection, the calibration solution was serially diluted to 0.01 μg/mL and each concentration level was analyzed five times.
The LOD was defined as the lowest concentration of the marker within the linear range of a quantification curve.
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