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We investigated the concentration range of probe DNA from 10 to 0.001 μM, finding an optimal value of 0.03 μM, approximately corresponding to ~0.9 probeol/cm2of probe DNA immobilized onto the silicon substrate.
It is demonstrated that when more than one molecular bond might rupture during the pulling measurements there is a noise-limited range of probe velocities where the kinetic parameters measured by force spectroscopy correspond to the true energy landscape.
Numerical results compare favorably with previous numerical and analytical solutions for a wide range of probe radius to Debye length ratios, probe potentials, and electron to ion temperature ratios.
Base oils used in the formulation of SBM had essentially no effect on the imbibition behavior of MXW-F, whereas exposure of MXW-F cores to two kinds of emulsifier resulted in persistently suppressed imbibition for a wide range of probe oils.
Distribution of correlation coefficients has been studied for the range of probe radii for every structure in the data set.
These results indicated that efficient fluorescence quenching exists between the terminally labeled fluorophore and quencher over a wide range of probe lengths.
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The device can secure a wide range of probes, within the limitations of size and nature of the probe.
We show that mechanically induced calcium signaling in the endothelial networks is dynamically regulated against a wide range of probing forces and repeated stimulations.
The mounting device has been used to hold a range of probes in a number of different animal models of intensive care including studies of brain death, traumatic brain injury, and sepsis.
Despite the wide range of probes commercially available for interphase fluorescence in situ hybridisation (FISH), the supply of locus-specific probes is limited to genes or chromosomal regions commonly altered in genetic diseases or during carcinogenesis.
Activity coefficients and interaction parameters for a range of probes have been measured and allow us to determine the nature and origin of the thermodynamic interactions in the systems.
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