Only DNA fragments in the range of the λ ladder (50 1,000 kb) were included in the analysis.
To obtain base-paired polymers with a wide and tunable range of structures (i.e., maximum ladder distances), we implement the following strategy.
To determine the lower fluorescence unit limit for reproducible quantitation of oligosaccharides, a dextran ladder comprising a range of different quantities was analysed.
These initial cycles were followed by 35 cycles at 94 °C for 30 s (lowest Tm –1 °C) for 1 min and 72 °C for 2 min, and a final elongation stage at 72 °C for 5 min. A classical ladder covering a range of 71 367 bp was added to each gel.
A ladder of proteins of a similar size range as the ladder observed in anti-CP5 blots was also seen for wild-type PglB samples on this blot (80 200 kDa).
Self-assembled films made of ladder [2- carboxymethylthio ethyl]-polysilsesquioxanes adsorbed on mica are stable within a wide temperature range (293–373 K).
Until recently, Peugeot had neglected the fifth rung of its model range ladder for many years; the popular 505, which followed in the tracks of the legendary 1968 504, died without replacement in 1992.
Note the presence of OX40L in lanes 7 and 9. (Lane 6, low range DNA ladder; lane 7, eYFP-C1 + OX40L insert; lane 8, eYFP-C1 plasmid; lane 9, eYFP-N1 +OX40L insert; lane 10, eYFP-N1 plasmid).
The samples as well as the molecular size markers (the BLUelf Prestained Protein Ladder covering a wide range of molecular weights from 3.5 to 245 kDa) were loaded into the wells.
In this system, we seek to optimize PAGE for a protein ladder spanning a mass range of 6.5 116 kDa.
