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Given the constraints of the short half-lives of the radioisotopes and the limited range of labelling agents that can be produced, it is usually not possible to use the same synthetic route that is used in the synthesis of the non-radioactive compound for which toxicity data would have been obtained.
After surveying a range of labelling conditions, protocols were established involving 5-min incubation at room temperature of aliquots of 5 80 μg protein at concentrations of 0.25 0.4 μg/μl.
The median and range of labelling indices were calculated.
Similar(57)
Treblers were superior to doublers, as trebler-labeled nucleic acids had fluorescence-signal-enhancing effects over a broad range of labeled DNA concentrations exemplified for the quenched single Cy3-fluorophore-labeled HSV-1 and non-quenched single Cy3-fluorophore-labeled CMV DNAs.
To determine the range and linear response of increasing amounts of mRNA, we hybridized a 500-fold concentration range of labeled RNA from cardiac tissue to the F. heteroclitus 384 cDNA metabolic microarray.
Availability of recombinant annexin A5 spurred synthesis of a wide range of labeled forms of annexin A5 to accomodate PS imaging with modalities such as optical, radionuclide and magnetic resonance imaging [ 71, 72].
We set labeling efficiency as 82% but also modeled a range of labeling efficiency from 51% to 100% as lower and upper bounds (indicated as 'Low L' and 'High limitsits).
The 293T-F population of DNA-bound TERT could not be modeled as a mixture of TERT monomer and dimer across the full range of labeling efficiency using the co-localization quantification, likely due to the substantial fraction of complexes with three or more TERT subunits.
The approaches have adopted a range of labels including "wellness," "consumer-directed health care" and the "medical home".
The subterranean bar and shop, with its low, arched stone ceilings, serves and sells a wide range of labels including artisanal cellars unknown outside of Georgia.
It is a British-based e-tailer that stocks a range of labels -- from heritage (Filson) to high-end (Prada) to Scandinavian (Henrik Vibskov) -- under one URL.
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