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66 stem segments from five temperate, diffuse-porous tree species (9 16 cm stem diameter, 100 cm stem length) were exposed to a wide range of flux densities by applying subatmospheric pressure (−50 to −650 hPa) analogous to natural flow conditions in the field.
In addition, the constraint parameters indicate the allowable range of flux values and are needed for convex solution space.
In FVA, one seeks to find a range of flux values on specific reactions that are compatible with maximal growth.
FVA was used to determine the range of flux values possible in order to maximize biomass production.
In defined medium (Chamberlain with glucose) 32% of the network reactions that span a range of flux values when biomass production was optimal were operational.
For overall reaction [3], the malate synthase reaction was blocked, whereas for overall reaction [4], the malate synthase reaction was substitutable, with a small range of flux values.
Similar(41)
We then demonstrate the procedure in Saccharomyces cerevisiae, using chemostats to generate a large flux/metabolite dataset, under 32 conditions spanning a large range of fluxes.
Many examples are shown for a range of fluxes and Reynolds numbers up to Re= 500 which illustrate the capabilities of our method.
Water chemistry associated with CO2 gas leakage into domestic groundwater resources maybe an important way to monitor the potential for further leakage to the surface over a wide range of fluxes, because the acidity associated with dissolved CO2 will alter the ambient water chemistry.
Our values of CO2 fluxes to the atmosphere lie within the range of fluxes from the world's rivers, but fluxes from the carbonate dominated region are at the low end, while fluxes from the siliciclastic region are at the high end.
Flux variability analysis defines a feasible range of fluxes for each individual reaction [ 27].
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