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Voltammetric investigations into the film's behaviour in a range of buffer solutions (pH 2.0, 4.5 and 7.0) were performed.
A study conducted by Onagh et al. (2012) showed that the landslide hazard occurred within the range of buffer zone created as of 300-600 m, 600-1000 m and 1000-1500 m.
The pH stability was determined by incubating rAaBGL1 (61.3 nM) in 100 mM various pH range of buffer (1.8 3.2, glycine-HCl buffer; 3.4 6.0, sodium acetate buffer; 6.5 7.3, sodium phosphate buffer; 7.2 9.1, Tris HCl buffer; 8.8 10.5, glycine-NaOH buffer) at room temperature for 1 h.
The optimum pH was determined by incubating rAaBGL1 (6.13 nM) with 1.5 mM pNP-Glc in various pH range of buffer (3.3 6.3, sodium citrate buffer; 6.5 7.3, sodium phosphate buffer; 7.0 8.9, Tris HCl buffer; 8.8 10.7, glycine-NaOH buffer) at 37°C for 10 min.
Throughout the range of buffer pH tested, the product e.e. remained above 99%.
With this approach, many resin aliquots could thus be studied rapidly to test a wide range of buffer compositions.
Similar(51)
Samples were incubated and arrays washed with a range of buffers at different pH (WCX/CM10 pH4-7, SAX/Q10 pH6-9).
Thus, results should not be very sensitive to variations within this range of buffers.
After testing a range of buffers, PBST with a pH of 7.4 was chosen as the most optimal buffer.
By varying the amount of each salt, a range of buffers can be prepared that buffer well between pH 5.8 and pH 8.0.
The technique is particularly useful as the reagents will react in a very wide range of buffers and conditions, so the secondary structure can be probed under exactly the same conditions as those used to probe the tertiary structure.
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