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It also shows that lowering the affinity for CD1d led to poor induction of PLZF and the iNKT lineage, suggesting that iNKT development is constrained by a limited range of affinity.
Despite a narrow range of affinity for BCMA, dramatic differences in CAR T cell expansion were observed between unique scFvs in a repeat antigen stimulation assay.
They allow direct observation of molecular interactions in a wide range of affinity and enzymatic assays and act as electron mediators.
We therefore conclude that genistein is able to bind to all three zebrafish estrogen receptors with a comparable range of affinity.
The development of a wide range of affinity tags has greatly simplified the separation of recombinant proteins from crude extracts.
In addition, B cells recognize antigen over a considerably wider range of affinity values (KA = 10 10 M−1) than do T cells (KA = 10 10 M−10 10
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The TCRs exhibited a wide range of affinities (KD values of 80 μM to 5 nM).
Bioinformatic analysis of the data reveals that clustered binding sites, spanning a broad range of affinities, best predict occupancy in cells.
A correlation between binding affinity and biological activity was found, albeit not across the whole range of affinities.
The 2C interaction with H-2Kb-dEV8, H-2Kbm3-dEV8, H-2Kb-SIYR, and H-2Ld-p2Ca spans a range of affinities from Kd = 10 -4) to 10 -6 M for the syngeneic (H-2Kb) and allogeneic (H-2Kbm3, H-2Ld) ligands.
Whereas CB2 binding did not vary greatly among all of the samples, the CB1 binding showed a wide range of affinities.
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