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The range of absorbance was at 384 nm.
The samples were diluted to stay in the linear range of absorbance (0.03 0.3).
The activity of FXa was determined based on the rate of hydrolysis of S-2765 from the linear range of absorbance at 405 nm over time.
For some of the substrates (most notably 12-pNCA and 2PE), many of the P450 variants were sufficiently active to either saturate the substrate or exceed the linear range of absorbance readings.
Then, CXCL8 and CCL2 levels were determined by ELISA in conditioned medium (CM), using standard curves with rhCXCL8 or rhCCL2 Catt. # 200-08 or # 300-04, resPeproTech; PeproTech), at the linear range of absorbance.
Of the 419 serum samples analyzed, a representative WU ELISA result of 29 serum samples in the 3-year age group is shown in Figure 2, panel A. A range of absorbance intensities were observed; 17 samples were above the cutoff value.
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Moreover, the absorbance of the prepared electro-devices in solar irradiation range was confirmed by UV vis spectroscopy characterization.
Although these results showed that WB-positive plasma samples from different clinical groups had a wide range of OD492 absorbance when assayed by ELISA, the median absorbance of positive samples of HUS group was higher than the NHC group (HUS = 0.51, n = 35; NHC = 0.40 n = 23; p<0.01, Wilcoxon Signed Rank test).
The range of spectral absorbance was between 0 and 6 which is related to air, to which 0 absorbance was assigned.
The H3K4me3 ChIP yielded an amount of DNA below the effective range of standard absorbance or fluorescence assays.
For the VZV IgG assay with modified assay conditions, the linear range of the absorbance values without denturant extended from approximately 0.200 to 2.800.
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