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The bin distance was similar to that generated by RAD-Seq (0.69 cM) [ 5], but was shorter than that maps constructed using randomly selective amplification markers mainly (1.86 cM) [ 24] or the specific length amplified fragment sequencing (SLAF-seq) technology (1.20 cM) [ 4].
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Randomly selected transformants were isolated on selective minimal medium agar.
Additionally, isolated DNA from randomly selected clones which had not been grown under selective growth conditions was used to perform PCR experiments.
The selective harvesting was repeated again when 50% of the randomly selected fish were mature.
Three of the four fecal samples containing cefadroxil resistant, randomly selected, E. coli also generated ESBL producing bacteria in the ESBL selective screen (E. coli with sample ID 63638, 63654, and 63725; Table 2).
Twenty randomly selected successful transformants with pPK2BarGFPD were consecutively sub-cultured on agar plates without the selective agent.
Enzyme combinations were randomly selected from Tab. 2. PCR was simulated with primers containing one to three selective nucleotides (total for both primers).
Instead, a subset of subcarriers can be randomly selected in each symbol, which can warrant a frequency diversity effect over a frequency-selective fading channel.
Plasmids were isolated and analyzed from 5 randomly selected mercury-resistant colonies from cultures grown during more than 70 generations under non-selective conditions.
If the sequence has been randomly generated, selective enrolment can occur if the next assignment is known before allocation.
Ten clones were taken randomly from each selective plate to identify genomic regions contained within plasmid DNA and confirm resistance phenotypes.
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