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The difference between the alignment scores is divided by the SD of the randomized alignment score distribution where the scores greater than 6 are indicative of homology between two sequences [39] [41].
The randomized alignment used the same tree as the myoglobin alignment.
In particular, the mean distance of alignments to the pseudoautosomal regions was not closer than 95% (n = 1000 replications) of simulated randomized alignment positions.
Briefly, the short-reads were converted to SAM files and mapped against the reference segment using a randomized alignment order to avoid mapping bias.
These results were similar for both mating-type chromosomes, but with the a1 chromosome approaching significance with the mean distance to pseudoautosomal regions being closer than 91% (n = 1000 replications) of simulated randomized alignment positions.
Alignment quality was assessed using average parsimony score (Fitch 1971) in sliding window analysis, where we compared the average score of the alignment obtained with the average score of a randomized alignment with gap retention.
Similar(54)
Since there is still no way of randomizing alignments preserving dinucleotide content, we cannot control for this effect.
To control for sampling error, full-length alignments were randomized by permuting the alignment columns after which the domains were extracted using the same coordinates as in the original alignment, and the distances were calculated as indicated above.
We removed all exonic sequences from the alignments and then randomized non-gapped columns in each alignment.
To assess over-fitting, phases beyond 40 Å in the tomogram were randomized and the last alignment iteration was repeated (Chen et al., 2013).
While ninein depletion had no effect on chromosome alignment, cenexin depletion randomized the distribution of unaligned chromosomes.
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