Sentence examples for random template from inspiring English sources

Theoretically, this random template control contains all possible ligation products in equimolar amounts.

Thus, our proposed CfERV amplification scenario favors a random template master gene model [26] over a strict master gene model [27].

In order to normalize the amplification efficiency of different primer sets, the PAC185 [15] plasmid containing the human β-globin locus was used for preparation of the random template control (RTC).

The diversity of CoVs is a result of the infidelity of RdRp which make CoV genomes especially plastic, a high frequency of homologous RNA recombination due to their unique random template switching during RNA replication, and their large genomes.

However, none of the random templates were as active as wild-type promoter.

For example, the time plotted for the range [10, 10) is the average time for all the random templates that produce between 100 and 1000 motifs. We find that the average running time for RISO across all extracted motifs is 120.7s, whereas for EXMOTIF it takes 88.4s for reporting only the supports, and 91.3s for also reporting all the occurrences.

Unlike ETA, this is done by choosing multiple semi-random templates of just three residues, biased towards conserved, non-hydrophobic, structurally neighboring residues with minimal overlap with other chosen templates.

The Monte Carlo effect is described by small, random and template concentration dependent differences in amplification efficiency.

Under physiological conditions, the HCV-polymerase revealed the highest affinity to the random ssRNA template.

The library construction was designed, and random oligonucleotide templates were synthesized as a single-stranded 88-mer with the following sequence: 5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3', where the central N30 represents random oligonucleotides based on equal incorporation of A, G, C, and T at each position [37], [39].

First strand cDNA synthesis was performed in duplicate in a 20 μl reaction volume, with 1 μg of total RNA as the template, random primer (random hexamer, 100 pmol), and M-MLV reverse transcriptase (200 U, Invitrogen, Karlsruhe, Germany) according to the manufacturer's instructions.