Sentence examples for random sequence pre-miR from inspiring English sources

A FAM-labeled, random sequence pre-miR was used for monitoring transfection efficiency by flow cytometry.

Pre-miR negative control 1 is a random sequence pre-miR molecule that has been extensively tested in human cell lines and tissues and validated to not produce identifiable effects on known miRNA function (http:// www.ambion.com).

In addition to a random sequence, miR-29b, miR-150, and miR-383 were employed as negative controls.

Downregulation of miR-146a activity was carried out by transfecting AC16 cells with 50 nmol/l human anti-miR-146a inhibitor, using a random sequence anti-miR molecule as negative control (Life Technologies).

What is important is the difference between pre-miRs and randomized pre-miR sequences, in terms of Standard Deviations - not the fold difference in hits.

The difference is in part explained considering that miRBase stem-loop sequences include the pre-miR and some flanking sequence of the presumed primary transcript, whereas FindMiRNA predictions describe only the putative pre-miR sequences.

For each sequence type, 715 sets (one for each pre-miR) of 1,000 random sequences of the same length were produced and the co-location with TFBS sequences was determined.

The number of motif hits was scored for each pre-miR sequence as well as for each of its randomized sequences.

Response: If you look closely at Figure 2 of the Saini et al paper, you will see that they characterized the regions UPSTREAM and DOWNSTREAM of the pre-miR sequence but they did NOT examine the pre-miR sequence itself!

In this case, each pre-miR sequence was randomly permuted 1,000 times (maintaining dinucleotide composition), and each permuted sequence was scored for TFBS motifs.

Because pre-miR sequences are so short, the possibility that the precursor sequence is included in an EST sequence is low.