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Probes were radioactive labelled with αP-CTP (Amersham) using Rediprime Random Labelling Kit (Amersham), including spermine precipitation of labelled DNA (Feinberg and Vogelstein, 1984).
Random labelling with radioactive chromium and cohort labelling using heavy nitrogen were considered.
Sparse, random labelling of individual cells is a key approach to study brain circuit organisation and development.
However, flaws associated with random labelling are significant and not all parameters controlling RBC survival in the model can be estimated with good precision.
Analyses were performed under the random labelling hypothesis and the critical values were obtained by using 199 permutations of a Monte Carlo test.
Probes specific for pHJO or pBeloBAC11 fragments were generated by random labelling with digoxigenin of sonicated purified BAC DNA.
Similar(45)
The significance of departure from randomness was assessed by random labeling performing 1000-time simulatoons to establish upper and lower confidence limits.
Oligonucleotide probe for detection was synthesized by random labeling of GFP gene with DIG-labeled nucleotides.
Both probes were gel purified and labeled with a Rediprime II random labeling kit (Amersham) with 50 μCi of αP-labeled-dCTP.
The novel method employs the two-step process (Figure 1): 1) preparation of 89Zr-labeled p-isothiocyanato-benzyl-desferrioxamine (89Zr-DBN) and 2) random labeling of primary amines of cell surface proteins with 89Zr-DBN.
That is, we allow the algorithm to start with a completely random label configuration.
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