Sentence examples for random immobilization from inspiring English sources

We found that antibodies with oriented immobilization induce a greater resultant surface stress than those with random immobilization.

Biofunctional membranes normally involve the random immobilization of biomolecules to porous, polymeric membranes, often through the numerous lysine residues on the protein.

Different strategies of antibodies immobilization - oriented immobilization of specific antibodies fragments (frag-anti-gp51) on gold surface obtained after chemical reduction and random immobilization of intact antibodies (anti-gp51) on gold surface, were investigated.

Oriented immobilization of frag-anti-gp51 was found to be more suitable for the design of SPR-immunosensor for the detection of gp51 if compared with random immobilization of anti-gp51.

Herein, we demonstrate that the random immobilization of gold nanoparticles (AuNPs) on a material's surface generates the possibility to explore microbial adhesion in dependence of contact point densities at the biointerface between the microbe, i.e., Escherichia coli and the material's surface.

A comparison of the FAC analyses, which for all three columns showed excellent linearity (R2 > 0.999), revealed that both site-directed approaches yield better results than the random immobilization; the by far highest efficiency, however, was determined for the immunoaffinity column based on AviTag-biotinylated antibody.

The resulting affibody variants were used for random surface immobilization (through amino groups) or oriented surface immobilization (through cysteine or biotin coupled to the side chain of Lys58).

The hydrazide carriers presented in Table 2 were modified with glutaraldehyde to perform the purely random covalent immobilization via amino groups of the antibody.

Random antibody immobilization occurs in the case of hydrophobic sorption, as well as when carriers containing the aldehyde, iminoester, halogeneacetyl, or other electrophilic groups are used to react with protein amino groups.

In this study, the effect of random vs. site-directed immobilization techniques on the performance of antibody-based HPLC columns was investigated using a single-domain camelid antibody (VHH) directed against methotrexate (MTX) as a model system.

This article reviews the most recent advancements in random and oriented antibody immobilization approaches for SPR biosensing applications, with a special focus on the research that have been done to find universal linkers, which can allow the use of the same functionalized surface for different applications.