Exact(11)
Fasted and random fed plasma insulin concentrations were comparable between MLC Cre + and MLC Cre - littermate control animals (Figure 1C).
Measurement of plasma insulin concentration under fasted and random fed conditions revealed no significant differences between 8 week old male Alb Cre + and Alb Cre- control mice (Figure 4C).
While infusion did not significantly affect body weight (Fig. 4A), both FGF21 and FGF19v dramatically reduced blood glucose levels in both random fed and fasted mice (Fig. 4A and B), reduced circulating free fatty acid levels (Fig. 4C), and improved glucose tolerance (Fig. 4D).
We then investigated basal blood glucose level under fasting and random fed conditions.
The same trends of increased glycogen were observed in random fed mice.
Overexpression of ANGPTL8 did not alter random fed blood glucose, glucose tolerance or insulin sensitivity (Fig. 1f h; detailed physiological data are shown in ESM Table 3).
Similar(49)
We next assessed glucose levels in random-fed and fasted mice.
Blood glucose from random-fed Smn +/−mice was normal relative to age-matched controls (Fig. 6B).
Random-fed mice were administered with 0.75 U/kg of insulin intraperitoneally.
Serum was collected from random-fed 1-month, 19-week and 1-year-old mice.
For creatine kinase assay, blood samples were taken by peri-orbital bleeding in random-fed mice.
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