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Mannan endo-1,4-β-mannosidase (commonly known as β-mannanase) catalyzes a random cleavage of the β-d-1,4-mannopyranosyl linkage in mannan polymers.
Mannan endo-1,4-β-mannosidase [EC 3.2.1.78] (β-mannanase) is used to catalyze a random cleavage of the β-d-1,4-mannopyranosyl linkage (McCleary and Matheson 1986).
In contrast, random cleavage of DNA in necrotic cells will produce a diffuse smear upon electrophoresis of DNA.
In contrast, cells expressing Eph4ΔC (n = 19 embryos) lost the oriented cleavage pattern and exhibited much more random cleavage angles (Figure 7B).
Lilly et al. [26] proposed that the formation of higher order multimeric molecules may result from recombinational events or the random cleavage and fusion of replication intermediates.
Our results indirectly support the alternative explanation that multimeric molecules were produced by the random cleavage and fusion of replication intermediates.
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Many longer RNAs are also fully extended but random cleavages that occur during sample processing and less than perfect RT processivity lead to stops throughout the RNA.
Aside from the well-studied hydrolases, new research suggests that enzymatic attack on crystalline cellulose is initiated by the redox enzymes cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenase (LPMO) forming a bi-enzymatic system [ 17– 19], which introduces random cleavages into cellulose through monooxygenation [ 20].
This analysis is an approximate examination of the non-random cleavage patterns of cell-free DNA fragments along the genome.
Detail examination of the sequences and the subsequent experiments suggest that the sequenced RNAs are likely to be non-random cleavage products of tRNA.
We further investigate this non-random cleavage by examining sequencing-read coverage patterns along the genome using strand-cross correlation analysis [ 58].
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