Sentence examples for ram domain from inspiring English sources

Experiments using NICD1 deletion mutants indicated a role for the RAM domain in this process, and either Jagged1 stimulation or expression of a transcriptionally active form of RBP-J in 32D cells also promoted myeloid differentiation (Schroeder and Just, 2000).

NICD is subsequently translocated to the nucleus where it interacts via its RAM domain with the DNA-binding protein recombinant recognition sequence binding protein at the Jκ site (RBP-J, also named CSL or CBF1).

The Notch RAM domain, which contains the RBP-J binding region, was subsequently shown to be required for these Notch-mediated functions (Tan-Pertel et al., 2000), implying that Notch signals through the canonical RBP-J-dependent pathway to inhibit terminal differentiation and enhance survival of 32D myeloblast cells.

Chimeric human Notch receptors (Notch1, 2, and 3) were created by inserting the sequence encoding the DNA binding domain of Gal4 into a Notch cDNA previously deleted of the sequence encoding most of the RAM domain and the ankyrin repeat domain as described [35].

In contrast, smaller components of this RAM domain are sufficient to stabilize RNMT.

This could be evidence that the additional domains interfere with RAM domain mediated hetero-oligomerization which alters their function.

Using pCMV/Myc-NICD as a template, RAM domain-deleted (ΔRAM), ANK domain-deleted (ΔANK), TAD domain-deleted (ΔTAD), OPA domain-deleted (ΔOPA) and PEST domain-deleted (ΔPEST) mutant constructs were developed by site-directed mutagenesis.

To investigate the RAM domains governing nuclear entry, HeLa cells were simultaneously transfected with RAM siRNA to suppress expression of the endogenous protein and with pcDNA5-based constructs to express Fg-RAM WT and mutants.

The atomic model of the protein shows a homodimeric association of two polypeptide chains, each containing an internal repeat of a ferredoxin-like βαββαβ fold, as seen in the ACT and RAM-domains.

Therefore the RAD (RAM 1 55), the NR domain (RAM 56 90) and the QYP domain (RAM 91 118) are all critical for RAM function.

To achieve rapid and efficient selection of positive integrants, a retrotransposition-activated selectable marker (RAM) was introduced into intron domain IV (DIV) [33].