Second, the ETA pipeline strives to raise specificity.
Overall, the methodologies taken by other researchers to raise specificity may also lower the likelihood of clinical application because of the very limited material that is usually obtained in bronchoscopic or core-needle biopsies.
To raise specificity, ETA filters geometric template matches (i) by ET rank similarity (Kristensen, et al., 2006); (ii) by match reciprocity back to the original protein (Ward et al., 2008); and (iii) by the extent that a plurality of matches point to the same function (Kristensen et al., 2008).
In the second approach we included follow-up information and we found that nine of the 16 tumors formerly classified as false positive turned out to be true positive, raising the specificity to 83.7% for hTERT (see Table 4).
They found that the addition of MRS increased the specificity of the exam from 62.5% to 87.5%, and the further addition of the perfusion measurement raised the specificity to 100%.
Chromatograms were screened for any interferences peaks at retention times of MST or IS, MRM mode in the mass detector was used to raise the specificity of the current method.
In total, our mutagenesis efforts improved the catalytic activity of Dr-OPH toward ethyl and methyl paraoxon by 126- and 322-fold and raised the specificity for these two substrates by 557- and 183-fold, respectively.
Whereas a prototype had only been 80% accurate and was not scalable, here a speedy new matching algorithm enabled large-scale searches for reciprocal matches and thus raised annotation specificity to 100% in both positive and negative controls of 49 enzymes and 50 non-enzymes, respectively in one case even identifying an annotation error while maintaining sensitivity (∼60%).
The combination of BPW analysis with PCT measurement did not increase the sensitivity but raised the specificity to 94% [ 15].
Association of Bm2+Bm2′/eBm5+Bm5⩾5 with anti-SSA and/or anti-SSB raised the specificity to 99.3%, but reduced the sensitivity to 68.0%.
