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Companies have the option of running a Regulation D raise in parallel, working with accredited investors.
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These flasks were raised in parallel until confluence, when each was again split into two centrifuge tubes.
Flasks of uninfected and T. hominis infected RK-13 cells were raised in parallel for 7 days post inoculation.
The external standard consisted of proteins extracted from worms fed 100% N-labeled OP50 bacteria over three generations and raised in parallel with the experimental samples.
White-CantonS (WCS) controls and spastin 5.75 mutant Drosophila were raised in parallel at 24°C ('untreated') or moved to 18°C during either their larval or pupal stage of development, and differences in eclosion rates determined for each condition (Fig. 1A,B).
The visiting overseas clinical fellows (including Mike Nicolle, Hidenori Matsuo and Anna-Paola Batocchi) carefully compared the specificities of the T cell lines with clones they had raised in parallel against synthetic AChR peptides, rather than recombinant subunits.
The interest for the completeness of coverage, and thereby for OCT, raised in parallel to the concerns about the risk of late and very late stent thrombosis associated with DESs.
To quantify the effects of cooling, we determined eclosion rate, climb rate and lifespan for spastin null animals reared at 18°C, and compared them with values for untreated (non-temperature-shifted) mutant animals raised at 24°C, as well as with control white-CantonS (WCS) animals raised in parallel conditions.
Since there was no untreated control group raised in parallel with these animals and they were all treated at the same time, it is possible that there was some unidentified factor such as a subtle change in diet or stress that accounted for the microbiota change.
However, the sites in South Africa never started recruitment because of the premature termination of the study on 31 January 2007 due to safety concerns raised in a parallel CS trial [ 9].
Years of raising our kids, in parallel lock step with hers, while reading her work, helped us along more than once.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com