Sentence examples for raft analysis from inspiring English sources

For lipid raft analysis of mammalian cells, 11 DIV neuronal cultures were washed with PBS and harvested immediately in TBS containing 1% triton-X-100, phosphatase inhibitors (1 mM Na3VO4, 0.1 µg/ml okadaic acid) and a protease inhibitors mix (Roche, Mannheim, Germany) and then incubated on ice for 30 min. Lysates were centrifuged at 1,000 g and supernatants were collected.

The cleared supernatants, with additional aliquots of protease and phosphatase inhibitors, were used either for differential centrifugation or for raft analysis.

Biochemical raft analysis showed that inhibition of ADAM10-mediated proteolysis of hFasL decreased APL production, while simultaneously increased the amount of full-length FasL preferentially inside the raft fractions.

As it is possible that HCC and ANT tissues may differ in the respective structures of lipid rafts such as those reflecting the composition of resident proteins and that these differences could be elucidated with lipid raft proteomic analysis.

Alternatively, it is also possible that cells may differ in the respective structures of lipid rafts such as those reflecting the composition of resident proteins, and that these differences could be elucidated with lipid raft proteomic analysis.

By an approach combining immunoprecipitation of PrPC from lipid rafts and mass spectrometry analysis, we identify the tissue non-specific alkaline phosphatase (TNAP) as interacting with PrPC in membrane microdomains of both 1C115-HT and 1C11NE cells.

Mass spectrometric analysis of lipid rafts of AC showed the expression of 5-LO, whereas lipid rafts of LC did not.

Among these proteins, tubulin, actin, annexins, enolase, GAPDH, gamma-glutamyltransferase, and HSP 27 have been demonstrated to be derived from lipid raft by proteomic analysis in previous studies [40], [41], [42], [43], [44].

Advanced raft support, allowing the analysis of lipid values and raft-restricted computation of lipid distributions.

For time-history analysis, the raft foundation resting on the surface of soil, is modeled using ShallowFoundationGen command in the framework of OpenSees (2012).

"In the raft of data and analysis that can so often define our work, we must never forget that all of our numbers reflect the lives of real people".