In the present study, our objective was to use a mouse cell line stably transfected with pBMN Rae -1 GFP to show that cell-based immunization can yield hybridomas to produce mAbs against the glycosylphosphatidylinositol (GPI -linked protein Rae-1.
This suggests that the 52A anti Rae-1 manti Rae-1zes Rae-1–expressing cells and thus could be used to detect Rae-1 localization in tumAb cells.
Five RAE-1 proteins have been described which are encoded by 5 different rae-1 genes: α, β, γ, δ and ε.
The figure 1B represents the rae-1 genes.
We confirmed the presence of Rae-1 transcripts in embryonic tissues and detected Rae-1 transcripts in adult tissues (Fig. 2A).
We confirmed that the expression of Rae-1 transcripts is characterized by a circadian rhythm.
Moreover, we showed that all Rae-1 transcripts, whatever the used promoters, are circadianly expressed.
RAE-1 is constitutively expressed during embryogenesis, mainly in the brain [9] whereas, in adult BALB/c mice, Rae-1 transcripts are not detected except in the adult spleen and liver where very low amounts are found [1].
Rae-1 was highly expressed in the liver and undetectable in numerous parts of the brain except in the SVZ, the main area of neurogenesis, where Rae-1 was weakly but significantly detected.
