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Due to the lower sensitivity of chemiluminescent detection compared with radioactive systems, an additional hybridization experiment was performed with cytotypes BB, which had been shown to possess lower amounts of satellite DNA, using 1 μg and additionally also 3 μg of genomic DNA.
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Quantification is improving [ 15] and radioactive detection systems have a wide linear range, but the linearity of the widely used chemical systems today is not well defined.
There are separate classes of radioactive decay systems on which different isotopic dating methods are based; cosmogenic nuclides (e.g. C-14, tritium and Cl-36), the uranium/thorium-series disequilibria systems (e.g. the 230Th-232Th and 210Pb methods), long-lived systems (e.g. the U Pb, K Ar and Ar Ar methods), and radiation damage methods (e.g. thermoluminescence and fission track methods).
It was also unexpected because conventional radioactive assay systems commonly detect de novo RNA synthesis by the HCV-polymerase within minutes to hours.
Two of the most commonly used non-radioactive readout systems for proliferation assays, the ATP bioluminescence assay and the fluorescent dye Alamar Blue assay, were initially tested as potency assays an anti-HER2 antibody.
However, non-radioactive labelling systems, such as the one described above, were shown to be 500 times more sensitive than the traditional method [23], [24].
The Radio-AllergoSorbent Test (RAST) uses a radioactive detection system: it is now rarely used but the name has persisted in common use.
With advances in mRNA profiling and detection techniques, we have established a novel and non-radioactive bioassay system using branched DNA (bDNA) technology for detecting protein-therapeutic neutralizing antibodies in patient serum.
The samples were then inoculated into the tubes within a MGIT 960 non-radioactive culture system (Becton Dickinson) where Mycobactin J and Egg yolk emulsion (20 µl) had previously been added to the 7H10 medium (Becton Dickinson).
The non-radioactive digoxigenin system (Roche Molecular Biochemicals, Indianapolis, IN) was used for labeling and detection of the transgene.
Filter screening was carried out using a non-radioactive detection system (ECL, Pharmacia Amersham/Pharmacia, USA) with X-ray film (Hyperfilm; Amersham/Pharmacia, USA) according to the manufacturer's instructions.
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