Sentence examples for radioactive assay from inspiring English sources

The recombinant PST did not use the alcohol substrate under the designed condition, and the sensitivity for AST activity was found to be comparable to that of radioactive assay as reported in the literature.

In this radioactive assay, Ndel1 itself did not exhibit any GTPase activity (Fig. 3A).

Consistent with data obtained with the radioactive assay, we also confirmed that Ndel1 shows no detectable intrinsic activity (Fig. 3B).

Non radioactive assay for Akt used similar protocol with only the radioactive ATP being replaced by 200mM of cold ATP and Gsk3β being used as substrate.

In the radioactive assay, the ProCA1-affi binding with 153Gd3+ was used to treat the cancer cells; the radioactivity of the cell pellets was measured by γ counter after washing 5 times.

Radioactive assay for Erk1/2 was performed using 10 µg of MBP with 9 µl of final immunoprecipitate bead in 15 µl reaction mix respectively containing 50 mM Tris Cl pH 8.0, 10 mM MgCl2, 50 µM ATP and 5 µCi of [γ-32P] ATP (3000 Ci/mmol).

A FabH assay using malonyl-ACP and a coumarin-based fluorescent reagent is described that provides a high throughput alternative to reported radioactive assays.

The final reaction condition was the same as described above for the radioactive assays.

Radioactive assays were carried out at 30°C under a single turnover condition with 5 nM substrate (see Text S1 for substrate preparation) and 1 µM DNAzyme in 70 mM HEPES (pH 8.0) containing 40 mM MgCl2, 0.001% Tween-20.

Although radioactive assays are sensitive, they are expensive and hazardous, requiring highly trained personnel.

JLB, RC and AY were responsible for radioactive assays and data interpretation.