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Radioactive analysis of soil extracts following sequential extractions showed that the 14C labeled residues of ZJ0273 were released from the amended soil BR upon planting.
By combining the fluorescent measurement with radioactive analysis, we determined that AMI-1 inhibits PRMT1 activity through the mechanism of blocking peptide substrate binding.
The colorimetric technique provided significant efficiency improvements compared with the processing time of both NMR and the radioactive analysis methods.
This probe could detect the tumor location with fluorescence imaging and assess the tumor-targeting efficacy of probe with radioactive analysis [ 32].
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Radioactive tracer analysis was another useful method to study ACTase activity in cell-free extracts.
Primers for specific regions (sequence available on request) were designed for radioactive PCR analysis of ChIP products.
Nuclear magnetic resonance spectroscopy, radioactive tracer analysis, and spectrophotometry were employed in conjunction to identify the properties of the enzyme activity and to validate the results obtained with each assay.
NMR spectroscopy was the most direct method to provide proof of ACTase activity; radioactive tracer analysis was the most sensitive technique and a microtitre-based colorimetric assay was the most cost-and time-efficient for large scale analyses.
The effects of CAA on ACTase activity were assessed confidently employing radioactive tracer analysis and, as shown in Figure 1, the observed product-inhibition of the enzyme activity was consistent with the inhibitory effects CAA had on H. pylori growth.
Fluorescence diffuse optical tomography (DOT) is an emerging deep tissue (>3 mm) imaging technique that has great potential as an alternative to radioactive tracer analysis for noninvasive detection and imaging of the sentinel lymph node.
Not surprisingly, we found that the cost, per sample, of performing this assay "in house" to be very cost effective and economical ($450.00 US at the Health Sciences Center vs $800.00 US per referral at Massachusetts General Hospital* for radioactive PCR analysis and $669.00 US at Mayo Medical Laboratories (MN) for fluorescent in situ hybridization analysisa).
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