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In his letter, Sang also asked for clarification of the radiation status of Hao, which was used as a forward base for the Pacific Experimentation Centre (CEP) and France's Atomic Energy Commission (CEA).
We conclude that irradiation of the AH produces a PB gene signature of radiation that reflects radiation status and can potentially be used to predict radiation status.
Importantly, PB gene signatures generated from TBI-treated mice failed completely to predict the radiation status of partially irradiated animals or non-irradiated controls.
These PB signatures predicted the radiation status of partially irradiated mice with a high level of accuracy (range 79 100%) compared to non-irradiated mice.
Recently, we demonstrated that PB signatures of TBI exposure were capable of predicting both radiation status and dose level of exposure in mice with 96% accuracy [9].
These results demonstrate that the total body model performs poorly in attempting to predict the radiation status of partially irradiated animals.
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Note that the variable used in the analysis is 'true_rt', the radiation therapy status as determined by interview with the subject and review of medical records (rather than 'rrt', the RT status recorded by the cancer registry and used for the counter-matching).
Patterns of gene expression could be identified in the PB of both male and female mice that appeared to distinguish radiation exposure status (Figure 2A).
In this study, we sought to determine whether PB gene expression signatures could be identified that predict radiation exposure status within a population that was heterogeneous for genotype, gender and time of sampling.
Counter-matching based on registry-reported radiation treatment status was used to improve the statistical efficiency of the study design.
The methylation patterns were qualitatively and quantitatively distinct in irradiated and unirradiated cultures, however there was no discernable quantitative or qualitative correlation among p16 promoter methylation and passage level or radiation exposure status.
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