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Several researchers have used radial profiling from MSA [49] to separate projections from different configurations, and this is also a common approach with icosahedrally-symmetric viruses.
For the present study, radial profiling was chosen as one way to approach the heterogeneity in LDL particles, since these particles have a normal distribution due to variable lipid composition and since this affects apoB-100 conformation.
Radial profiling provides spatial averaging.
Radial profiling allows phase noise reduction compared to single profiles while maintaining the flow profile shape.
Radial profiling was performed as described (Conduit et al., 2014b) using ImageJ.
We then centred concentric rings (spaced at 0.028 μm and spanning across 3.02 μm) on this centre and measured the average fluorescence around each ring (radial profiling).
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The radial profiles of (c) amplitude ratio and (d) correlation coefficient.
The average radial profile of magnitude is discussed.
Figure 6 Radial profiles of the background cold electrons at the equator of the simulation system.
The black circles represent the radial profile and the gray line depicts the smoothing spline.
Fig. 3. Radial profiles of Icont = (I8.8 + I12.4 /2 multiplied by r as a function of time.
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