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In maize, 199 RGLs have been identified using data-mining, modified amplified fragment length polymorphism (AFLP), and rapid amplification of cDNA ends (RACE) methods [ 25].
We further sequenced the genome of NC-WGP93C strain by using primer walking, 3′ and 5′ rapid amplification of cDNA ends (RACE) methods (3, 6, 7 ).
Rapid Amplification of cDNA Ends (RACE) methods were used to obtain the entire open reading frame for GSIIE sequences from Pseudochlorella sp. CCAP211/1A, C. luteoviridis, A. protothecoides and P. zopfii and partial GSIIB sequence from Pseudochlorella sp. CCAP211/1A.
By 5′ and 3′ RACE methods for cloned partial CSD sequences, total length of CSD (1882 bp and 1821 bp) from red sea bream and yellowtail was determined.
A selenium-dependent glutathione peroxidase cDNA was obtained from green mud crab Scylla paramamosain (SpGPx) by homology PCR technique and rapid amplification of cDNA ends (RACE) methods.
Wiles and Alters' ([2011]) study used intensive instruction to address specific hypothesized impediments to acceptance (e.g., false dichotomy between science and religion; relationship between evolution and race; methods in dating; origin of life; etc)., although in the end, the authors were unable to determine which educational experiences most influenced acceptance of evolution.
The amino acid sequence of HWTX-XI was also confirmed from its cDNA sequence obtained using 3'-and 5'-RACE methods (Fig. 1D).
To identify the whole length of kakusei cDNA, 5'-and 3'-rapid amplification of cDNA ends (RACE) methods were performed repeatedly using the SMART RACE cDNA Amplification Kit (Clontech).
The main limitation full-length cDNA or 5' RACE methods try to resolve is binding a known sequence at the cap site, so as to prime second-strand polymerization of the cDNA.
The transcription status of this mouse region was investigated by RT-PCR and RACE methods.
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