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Circadian race tube experiments were performed with the Inverted Race Tube Assay in accessions that lack the bd mutation [29].
However, the race tube assay requires a genetic mutation band (bd) to mitigate the adverse effects of CO2 accumulation [28].
Circadian regulated conidiation (banding) serves as an accurate phenotypic readout of the endogenous cellular clock and is measured with the race tube assay [1].
We used the inverted race tube assay to score the period and phase of progeny from the cross (Figure 5, Table S2).
We developed the 'Inverted Race Tube Assay' to directly measure the clock parameters in N. crassa accession lacking the bd mutation [29].
Race tube media contained 1× Vogel's, 0.17% arginine, 0.1% glucose, and 50 μg/mL biotin.
Similar(48)
Lifespan of P. anserina was determined in race tubes as previously described [16].
Race tubes were incubated in constant light for 24 hr and then moved to constant dark.
Growth rates were determined by growing strains in race tubes.
Freshly germinated spores were placed on race tubes with PASM.
The strains grew along 30 cm race tubes while exposed to the selective temperature.
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