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For Race, the expression is preferentially lost in the posterior in scb mutant embryos, whereas it is lost uniformly along the anterior-posterior axis in mew mutants (compare embryos classified as "weak" in Figure 1C).
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The apterous gene model in Hydra was found to be incorrect as 5' rapid amplification of complementary DNA ends (RACE) determined the expression of another exon containing the second LIM domain that was found to be encoded in the genomic sequence upstream of the predicted gene model.
Additional file 10: Real-time quantitative PCR and RLM-5′-RACE confirming the expression of other miRNA-targets potentially involved in litchi fruit senescence.
Third, 5'- and 3'-RACE confirmed the expression of the ZNF280BY type B and C. Finally, multiple alignment of promoter sequences indicated that ZNF280BY type A promoters are highly conserved (>98% similarity).
Using RT-PCR and 5′ RACE, we confirmed the expression of exon 1f in the hypothalamus and proximal exon P2 in the ovary.
But nothing will change unless more individuals choose to step into the spotlight, making their voices heard so others can understand that regardless of HIV status, race, religion or the expression of one's gender we are all flesh and blood, and we all have the right to happiness.
Our decision to use RACE to verify the expression and structure of these un-annotated genes was necessitated by their low level of expression and uncertain gene structures.
In the present study, the full-length cDNA sequence of a TCTP from Zhikong scallop Chlamys farreri (designed as CfTCTP) was cloned by rapid amplification of cDNA ends (RACE) technique based on the expression sequence tag (EST) analysis.
The RACE results revealed that the expression of these two NBS-LRR-encoding genes showed different expression pattern in leaves, seedlings, and flowers.
In contrast to integrin and collagen IV mutants, embryos lacking β-laminin show a variable expansion of the Race expression domain.
In previous cases, targets of miRNAs were identified by computational prediction in silico, and then using modified 5′-RACE-PCR to confirm the expression of target genes in pollen development [ 29- 31].
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