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Homologs of AP3 were cloned from select taxa (see Fig. 1) using reverse transcriptase polymerase chain reaction (RT-PCR) on floral RNA following the protocol described by Stellari et al. [ 27] and Kramer et al. [ 28]. 5' rapid amplification of cDNA ends (RACE) was performed on TroAP3 using 5' RACE system (Invitrogen™ Life Technologies, Carlsbad CA).
The CC had a central barn for weight classification with a curved race system and scale.
According to that study, 42% of specimens instrumented with ProTaper presented dentinal defects, along with 35% for Safesider and 25% for Race system.
This is a disappointment -- for our race system is much deeper than that.
The 5' rapid amplification of cDNA ends (RACE) system (Invitrogen) was used as per the manufacturer's instructions.
Total RNA was extracted with the RNeasy Mini RNA kit (Qiagen, Valencia CA) and the 5' RACE system (Invitrogen) was used.
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5'-RACE reactions were performed with the 5'-RACE System (Invitrogen) using primers GSP2 (5'-TTGTCCTCCAACTGCCTCAGA-3') and nested primer GSP3 (5'-CCAAGACAACAGCACCCCACAA-3').
The 3'-RACE (3'-RACE System, Invitrogen, Carlsbad, CA, USA) and 5'-RACE (Smart Race Kit, Clontech, Palo Alto, CA, USA) methods were subsequently carried out according to the manufacturer's instructions.
The chemically-synthesized oligonucleotide primers used in this study were listed in Table 3. Rapid amplification of 5'-cDNA element (5'-RACE) was performed to identify the transcriptional start sites for the two phz clusters by using Invitrogen's 5'-RACE system.
RACE (using 5′-RACE System; Life Technologies) was used to amplify 5′-exons.
3′-ends of these four fragments were isolated by applying the 3′-RACE System (Invitrogen).
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