Your English writing platform
Discover LudwigSuggestions(1)
Exact(32)
RACE product #12 was similar to RACE products #9 11, 13 15 except that YL-1 splices from exon 5 to TMOD4 exon 8 which then splices to exon 9 (Fig. 2).
The success of the RACE pipeline, defined as the frequency of obtaining RACE product(s) that mapped to an intergenic prediction (regardless of how well the experimental evidence agreed with overlapping predictions), is summarized in Table 2.
Following RNase H treatment, the RACE product was 1∶10 diluted and used as template for PCR.
The RACE product was enriched by reamplifying with the Abridged Universal Amplification Primer and OPCML-R, 5'-TATGGACCACTTGTCATTCC, cloned and sequenced.
The most distal RACE product (observed once at position −146) was at a position consistent with signals observed with primer S3.
The gel slices containing DNA with a size of about 60 bp (5' RACE product) and 87 bp (3' RACE product) were excised and the DNA fragments were purified using an agarose gel DNA purification kit (Takara, Japan), according to the manufacturer's instructions.
Similar(28)
Sequences were analysed by identifying overlapping regions between the partial fragments and the 5′ and 3′ RACE products.
RACE products yielded a complete coding class I sequence designated Amcr-UA.
It also included previously identified 5' Rapid Amplification of cDNA Ends (RACE) products [31].
Amplified 5' RACE products were cloned into TOPO pCR2.1 and sequenced from both ends with M13 primers.
The analytical gel was blotted onto a Hybond N membrane and the blot was hybridized with a RACE product-specific oligonucleotide probe (5′-GGAGTAGAAATGGAACTCCT-3′) labelled with [ γ-P]ATP (Perkin-Elmer).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com