Exact(2)
Full-length cDNA sequence for ERα was determined by 3′ and 5′ RACE procedures.
Based on the sequence information of the partial complimentary DNA (cDNA) of Foxl2, rapid amplification of cDNA ends (RACE) procedures were performed in order to isolate the 5' and 3' ends of the cDNA (SMART cDNA library construction Kit; Takara, Shiga, Japan).
Similar(58)
The partial cDNA sequence was then used to make gene specific primers for a rapid amplification of cDNA ends (RACE) procedure that provided repeatable 5′ and 3′ cDNA ends.
At the start of the race, all the cars lined up on the grid per FIA race procedure.
Prior to the RACE procedure, adapter-ligated cDNA was diluted fifty fold in TE-buffer and denatured by heating at 100°C for 2 min and thereafter placed directly on ice.
He was summoned by the stewards for ignoring post-race procedures and performing crowd-pleasing "doughnuts" with the car spinning in a circle of tire smoke at the end of the pit straight.
cDNA encoding the complete precursor protein of horse (Equus caballus) latherin was obtained by RT-PCR and 5'- and 3-RACE procedures using oligonucleotide primers based on the amino acid sequences of tryptic fragments derived by Edman degradation of latherin obtained directly from sweat-derived protein, purified as previously described [8], and mRNA obtained from skin samples.
The cDNA was subjected to 3′-RACE procedures to obtain full-length prepro-bombinin nucleic acid sequence data using a SMART-RACE kit (Clontech, UK) essentially as described by the manufacturer.
Unexpectedly, the 3′-RACE procedure using the same primer led to amplification of a cDNA encoding a preprobradykinin whose signal peptide region was identical to that of preprotemporin-1SKa except for the substitution Ser18 → Asn.
Using a forward primer that was designed from a conserved region of the 5′-untranslated regions of Rana temporaria preprotemporins in a 3′-RACE procedure, a cDNA clone encoding preprotemporin-1SKa was prepared from R. sakuraii skin total RNA.
Each time this happens, the race must restart; oddly, the racing procedure (placement, fouls, etc). is much slower than the creatures themselves.
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