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By examining overlapping regions from the RACE procedure, full-length sequence information was obtained for the bovine lysin homologue.
The partial cDNA sequence was then used to make gene specific primers for a rapid amplification of cDNA ends (RACE) procedure that provided repeatable 5′ and 3′ cDNA ends.
At the start of the race, all the cars lined up on the grid per FIA race procedure.
To extend this sequence we performed the RACE procedure, obtaining full-length A2ML cDNA (accession number EU689051).
Prior to the RACE procedure, adapter-ligated cDNA was diluted fifty fold in TE-buffer and denatured by heating at 100°C for 2 min and thereafter placed directly on ice.
The initial PCR amplification in each RACE procedure was followed by a nested PCR.
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Full-length cDNA sequence for ERα was determined by 3′ and 5′ RACE procedures.
Based on the sequence information of the partial complimentary DNA (cDNA) of Foxl2, rapid amplification of cDNA ends (RACE) procedures were performed in order to isolate the 5' and 3' ends of the cDNA (SMART cDNA library construction Kit; Takara, Shiga, Japan).
(a) Schematic representation of the RLM-RACE procedure.
The RLM-RACE procedure was modified to ensure that only transcripts with six or more adenosines at the 3′ end were amplified53.
There's also a free mode for taking out any car on any of the six available environments, and the pre-race procedure contains much of the complexity of the full Xbox titles, including assists, tuner set-up and practice options.
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