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By 5′ and 3′ RACE methods for cloned partial CSD sequences, total length of CSD (1882 bp and 1821 bp) from red sea bream and yellowtail was determined.
A selenium-dependent glutathione peroxidase cDNA was obtained from green mud crab Scylla paramamosain (SpGPx) by homology PCR technique and rapid amplification of cDNA ends (RACE) methods.
We cloned the AmI2′H gene by employing RACE methods and modified the gene sequence to facilitate protein expression and increase protein solubility.
Wiles and Alters' ([2011]) study used intensive instruction to address specific hypothesized impediments to acceptance (e.g., false dichotomy between science and religion; relationship between evolution and race; methods in dating; origin of life; etc)., although in the end, the authors were unable to determine which educational experiences most influenced acceptance of evolution.
Using RT-PCR and RACE methods, we cloned the full-length cDNA sequences of the CkTLP gene (GenBank accession number: GU067481).
The main limitation full-length cDNA or 5' RACE methods try to resolve is binding a known sequence at the cap site, so as to prime second-strand polymerization of the cDNA.
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The missing sequences were amplified by the 5' RACE method [ 75] (5'/3' RACE kit, Boehringer Mannheim, cat. no. 1734792).
Its partial cDNA was obtained by using 5′ RACE method.
The full-length cDNA sequence of EhAACT was obtained by RT-PCR Using RACE method (GenBank Accession No. KP995935).
Two fragments, a 286 bp long 3′- flanking region with poly (A) and a 519 bp long 5′- untranslated region (UTR) were obtained by RACE method respectively.
The identification of miRNA processing intermediates in plants has mostly relied on a modified 5′ RACE method, designed to detect the 5′ end of uncapped RNAs.
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