Sentence examples for race method from inspiring English sources
The missing sequences were amplified by the 5' RACE method [ 75] (5'/3' RACE kit, Boehringer Mannheim, cat. no. 1734792).
Its partial cDNA was obtained by using 5′ RACE method.
The full-length cDNA sequence of EhAACT was obtained by RT-PCR Using RACE method (GenBank Accession No. KP995935).
A full length novel H+-pyrophosphatase gene was isolated from halophytic grass Leptochloa fusca using RT-PCR and RACE method.
Two fragments, a 286 bp long 3′- flanking region with poly (A) and a 519 bp long 5′- untranslated region (UTR) were obtained by RACE method respectively.
A cDNA, named aleh1, was obtained by the RACE method following PCR with degenerate primers designed based on amino-acid sequences of FEHs from other plants.
A 910 bp of PCR product was obtained applying 5′-RACE method.
An open reading frame encoding a cysteine protease inhibitor, cystatin was isolated from the buds of sugar beet monosomic addition line M14 (BvM14) using 5′-/3′-RACE method.
The 5'- and 3'- ends of the ts-pchtp mRNA were determined by RLM-RACE method.
The transcriptional start sites of each gene cluster, phzA1-G1 and phzA2-G2, were determined by a rapid amplification of 5'-cDNA element (5'-RACE) method and a long non-coding region of 338 bp and 201 bp was found between the transcriptional start point and the predicted translation start point in the two gene clusters, respectively (Figure 1A).
Using the modified 5′-RACE method, we confirmed that SmAGO1 and SmAGO2 were targeted by S. miltiorrhiza miR168a/b and miR403, respectively.
