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For RACE analyses, polyA-enriched mRNA was isolated using the Dynabeads DIRECT kit (Invitrogen).
We also performed RT-PCR, 5' RACE and 3' RACE analyses.
The two contigs were used to design sxtA1 and sxtA4 primers for genomic amplification, RACE analyses and sequencing.
Furthermore, RACE analyses of A. tamarense strain CCMP1771 revealed that sxtA1 and sxtA4 were transcribed in this supposedly non-STX-producing strain (data not shown).
For example, some regions of the sxtA transcripts were also not recovered in the 454 dataset, but only obtained through RACE analyses (see above).
The ADAMTSL2 transcript was assembled from overlapping RT-PCR products and Rapid Amplification of cDNA Ends (RACE) analyses (Genbank accession no. GU220770; Table S5).
Similar(41)
A wealth of online data about competitive running makes post-race analyses relatively easy.
Most pre-race analyses identified Ivan Basso, Cadel Evans, and Carlos Sastre as the major favorites to win the overall classification, with Evans perhaps the consensus pick.
The team did not have any rider highlighted as a favorite entering the Giro di Lombardia; pre-race analyses held Gilbert as the man to beat.
The Schleck brothers were again the squad leaders for the remaining Ardennes classics, La Flèche Wallonne and Liège Bastogne Liège. Pre-race analyses mentioned them as contenders for both races.
Firstly, 3'- and 5'-RACE analyses were performed.
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