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PAC and RAC (1 mg in 0.5 ml), which were stored at –80 °C in reduced form, were desalted on Zeba column.
In contrast, the cells coexpressing dominant negative Rac 1 (Rac1Asn17) or dominant negative Rho A (RhoAAsn19) showed no change of sensitivity to 5-FU.
Isoprenylation of the low molecular mass GTP-binding proteins rac 1 and rac 2: possible role in membrane localization.
A similar COOH-terminal amino acid sequence, Cys-Xaa-Ali-Xaa, exists in the ras-related GTP-binding proteins rac 1 and rac 2. To determine whether these proteins were similarly modified, COS cells were transfected with rac 1 and rac 2 cDNA and expressed proteins were labeled with [3H]mevalonic acid.
Although similar amounts of Rac 1 were loaded, androgen-induced Rac 1 activation is even more evident in FAK-/ fibroblasts (Fig. 4A).
Possible candidate is DGKζ because it promotes neurite outgrowth via Rac 1 [16].
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Activation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins.
Rac-1 and its enantiomeric isomers were evaluated against important bacteria responsible for dental caries.
The enantiomer (R -1 and R -1mand (racemateerac -1thesized frac -1eir corresponding Ni(II) glycine equivalents.
In block copolymerization of rac-1 using the poly-l-150 as the macroinitiator, the polymerization proceeded in enantiomer-selective manner.
A preparative resolution of 100 g/L rac-1 was accomplished with high enantioselectivity (E = 92) using a variant of Bacillus megaterium epoxide hydrolase (BmEHF128T).
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