Exact(17)
In culture, bumetanide and ouabain were equipotent in reducing by approximately half the K+ uptake of quiescent, rabbit lens epithelial cells under control, iso-osmotic conditions, indicating a cell-culture induced up-regulation of the cotransport activity by an undetermined mechanism.
Overall, these results suggest that the posterior fiber cells may lack ACh receptors, which are clearly present in the lens epithelium that covers about two-thirds of the rabbit lens surface area, and indicate that an ACh-evoked Ca2+ signal does not spread throughout the epithelial layer.
The dogs' lenses showed the same type of dissolving pattern as the human and rabbit lens cells.
We next tested whether reducing AhR levels by siRNA or increasing AhR levels using plasmids encoding AhR and ARNT would affect αB-crystallin levels in cultured rabbit lens αTN4 cells.
The rabbit lens is suitable for several reasons.
Cx46 is upregulated by hypoxia in rabbit lens epithelial cells, which naturally express Cx46.
Similar(43)
To complement previous results, 86Rb+ uptake experiments were done in a chamber design that limited exposure of the radiolabel to specific surfaces of rabbit lenses under short-circuit conditions.
First, both human and rabbit lenses possess branched suture patterns.
Statistical calculations (Student's t-test) were performed to determine if scatter in aged rabbit lenses was significantly different from adult rabbit lenses.
Additionally, structural parameters indicated that age-related compaction may have occurred in rabbit lenses.
However, these were not consistently present in all aged rabbit lenses (5 of 8).
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