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It can be noted from both the MA and the volcano plot that significantly DE genes can have quite low expression ratios as some of the most significantly DE genes exhibit less than a twofold difference, an otherwise commonly used ratio cutoff level of significance.
However a significant number also have quite low expression.
The consistent but quite low expression of UBQLN4 is, by comparison, dwarfed.
Generally, a small number of categories of mRNA showed high abundance, whereas the other majority had a quite low expression level.
However, as we show in Figure 2 figure supplement 2, we can accurately measure YFP expression even at quite low expression levels.
Another study in mice showed that the ligands Wnt4, 5a, 5b, 10b, and also Dkk1 and sclerostin were upregulated in a similar pattern, with a peak around day 10 but quite low expression during the first days.
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In oesophageal tumour cell lines, the average expression level of GNG7 was quite low (relative expression: 0.11), similar to results obtained with clinical tumour samples.
We observed that the expression of Dis3l2 mRNA at the wound sites increased from 18 h to 2 days post-amputation with quite a low expression in intact worms, indicating that Dis3l2 was induced by tissue-missing injury (Fig. 5E).
In our present study, we have employed 40 cases of HCC tissues and their controls, although quite limited, observing that low expression of let-7g was significantly associated with poorer overall survival.
Wild type and d18-dy also showed similar expression pattern of miR172: the expression was quite low in 2nd leaf, and increased dramatically toward the 7th leaf (Figure 3B).
Our results also indicate that p68 expression is quite low in MCF-7 cells as compared to highly metastatic MDA-MB 231 and mouse 4T1 cells with reduced expression or absence of E-cadherin, which is corroborated with the previous findings in breast tumours and cell lines [ 35].
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