Flashes of any size occurred amidst a background of quiescent mitochondria, suggesting that flashes do not simply propagate to adjacent mitochondria under resting conditions.
Importantly, once Prospero levels returned to normal background levels, the resulting quiescent neuroblasts started proliferating again, confirming that neuroblasts exposed to a short pulse of Prospero do indeed become quiescent.
According to Eq. (7), the RTL score varies between −1 and 1, where a RTL score of <0 or >0 implies a quiescent or activation stage, respectively, when the background RTL score = 0.
Close examination of each quiescent interval reveals a subtle growth in background activity that commences about 30 s prior to avalanche, and that this activity has spectral energy that initially extends to ∼20 Hz, but drops to lower frequencies as onset approaches, forming a characteristic "down-chirp" in the spectrogram of Fig. 9(b).
Her medical background included migraines, hypertension, mild quiescent psoriasis, and previous depression.
Importantly, the low background of EFGP-Luciferase in quiescent cells together with the progressive accumulation of EGFP-Luc signal, appear to provide conditions that allow an amplification of events, like serum stimulation, that trigger c-Myc transcription.
Despite the relatively non-hazardous nature of such prolonged hydrothermal background activity, a profound knowledge of quiescent activity of individual volcanoes is necessary to understand the short- (daily) and long-term (seasonal, yearly) baseline behavior from which to deviate when evolving towards a state that causes concern.
Surveillance data have previously demonstrated that in one or two seasons a usually quiescent serotype may cause an outburst of clinical disease superimposed on background, area-endemic enteroviruses (24 ).
The system is based on an integrative vector that facilitates inducible expression of specific genes in a transcriptionally quiescent locus and engineered cell lines that constitutively express the T7 RNA polymerase and tetR genes from an episomal background.
