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Quick washing and chilling, along with innovations in packaging and store displays, also help to keep food fresher for longer, both on the supermarket shelf and in our fridges at home.
After 10 min of incubation/attachment of the exosome sample to the formvar-coated grid, a quick washing was performed with PBS followed by 15 min incubation in blocking buffer (0.8% BSA and 0.1% fish skin gelatin in PBS).
For the PCR-RFLP assay, a 1.2 mm disc punched out of the FTA card was used as a template after quick washing as reported previously [ 4].
After quick washing in PBST (PBS and 0.1% Tween 20), the membranes were blocked in 5% non-fat dry milk in PBS for at least 2 h and then incubated with primary antibodies (anti-MUC4, both activated form and total of HER2, FAK, Akt, ERK, p38 and β-actin).
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But it does mean it might be worth giving them a quick wash.
Basic preparation is simple – a quick wash followed by a top-and-tail.
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CEO of Professional Science Editing for Scientists @ prosciediting.com