Sentence examples for quick protocol from inspiring English sources
This extremely simple and quick protocol gave quantitative labelling and required no post-labelling purification or harsh conditions such as low pH or elevated temperature that might denature the protein, and is a suitable basis for future development of a simple, one-step kit-based GMP labelling procedure for proteins.
Since the histopathology of frozen tissue is closer to that of fresh tissue and since frozen sections can be produced more quickly than paraffin-embedded sections, SYL3C immunostaining of frozen sections is a quick protocol that is easy to implement.
Similarly to what we have shown for other organs, we were able to establish for the lung a quick protocol that avoids harsh chemicals that were deemed a necessity for complex organs thus far.
We devised a simple and quick protocol for the purification of dye contaminants from protein samples, which consists of binding the protein of interest to the Ni NTA column, and washing the column with a large amount (100 CV) of buffer.
Further, different charging protocols are analyzed and a model based methodology is suggested to design an efficient quick charging protocol.
By using a quick purification protocol, in which the hexahistidine tag was added at the C-terminal end of the protease, the recombinant CHY1 protein could be purified in a single step on an Ni-NTA column as a mixture of 19.5- and 15-kDa mature active forms and did not require further activation/maturation steps.
Purified DNA was bidirectionally sequenced by BaseClear B.V. (Leiden, The Netherlands) according to their quick shot protocol (www.baseclear.com).
DNA was extracted following the Quick CTAB protocol.
Good survival rates are probably related to a special quick response protocol and trained teams.
We isolated genomic DNA from fin-clips using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA) or the quick extraction protocol [ 58].
Amplified products were analyzed by 1% agarose gel electrophoresis (AGE), and PCR products were cloned directly into pCR Blunt II TOPO vector (Invitrogen™/Life Technologies) using the quick ligase protocol (NEB).
