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Considering the short physical half life of 213Bi (45.6 minutes) a quick internalization of the carrier is essential.
The results of the internalization experiments demonstrated a quick internalization of the peptide at 37°C, which thereafter decreased with progression of time.
In particular, Chrastina et al. demonstrated a quick internalization of 125I-labeled monoclonal antibody M75, which is specific for human carbonic anhydrase IX, in human colorectal carcinoma cells [16].
Treatment of EU12 μHC+ cells with low concentration of goat anti-human IgM antibody F ab′ 2 fragments (2 μg/mL) resulted in quick internalization of cell surface BCRs, which could be seen as soon as 1 min after adding the antibodies.
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These results can be related to the fast internalization of PTX-NS, that ensure a quicker and higher drug concentration available inside the cells.
In the EU12 μHC+ cells, crosslinking BCRs with anti-IgM antibodies resulted in quick BCR internalization, which can be seen as soon as 1 min).
Agonists at MOP cause fast receptor internalization with internalization half-times 2 5 min (Fig. 1D).
This novel assay is a quick and reliable method to evaluate the internalization of apoptotic cells.
Cells were incubated with transferrin-Alex 594 instead, placed on ice after internalization, washed three times with cold PBS, and surface bound transferrin was removed by a quick acid wash (0.2 M acetic acid, 0.5 M NaCl).
The economist Joseph Farrell called this the "internalization of complementary efficiencies".
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