Sentence examples for quick chilled from inspiring English sources

The first step used 5 µg RNA, random hexamer, dNTP mix, and ddH2O to a final volume of 12.5 µl incubated at 65°C for 5 minutes then quick chilled on ice.

Total RNA (800 ng) was incubated with each of 10 gene-specific primers (Additional file 4) at 65°C for 5 min and quick chilled on ice.

Reaction mixtures were incubated at 37°C for 50 min and at 42°C for 15 min. The RT reaction was terminated by heating at 95°C for 5 min and subsequently quick chilled on ice.

Equal masses of cDNA from males and females were combined in a 1 3 volume with hybridization buffer (50% formamide, 5× SSC, 0.1% SDS, 10 μg calf thymus DNA) and denatured at 90°C for 4 min, quick chilled, and injected into a Lucidea Slidepro automated hybridization chamber (Amersham) containing a microarray slide pre-hybridized according to manufacturer's instructions.

Hybridization targets were heated to 95°C, quick chilled on ice, and kept at 50°C covered from light until applied to arrays.

Total RNA (less than 1 μg each) was added to 100 ng oligo-dT and 0.4 mM dNTPs, incubated at 65°C for 5 min and quick chilled.

We changed into our evening outfits, already feeling the quick chill of the night.

When the character seduces a woman in a hotel room, he creates the longer-lasting feeling of suspense than just a quick chill.

The shell will assume tremendous importance as a ritualized symbol of an order that cannot hold, and its summoning blast sends a quick chill.

As a quick chill descended on Boston in the fall, "even the faces of the squirrels looked different as they scurried around collecting food".

It involves slow cooking in a vacuum-sealed pouch, placed either in an oven into which steam is pumped or in a water bath, followed by quick chilling.