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Moreover, the temporary reduction/suppression of the separation bubble observed when the incoming wake reaches the separated flow region has been found to be driven by linear stability mechanisms, which promote a quick amplification of the high frequency velocity fluctuations carried by wakes.
Five hundred ng of total RNA from each control and PPAR agonist-treated cell culture were separately reverse-transcribed into double-strand cDNA by the Moloney murine leukaemia virus reverse transcriptase and amplified for 2 h at 40°C using Quick Amplification Labeling Kit (Agilent).
Briefly, 200 ng of RNA was amplified by conversion to cDNA, and subsequently to labelled cRNA, with the Low-Input Quick Amplification Labelling kit (Agilent Technologies).
Briefly, 200 ng of RNA per subject was converted to Cyanine-3 labelled cRNA with the Low Input Quick Amplification Labelling kit (Agilent Technologies).
One hundred nanograms of RNA of non-diapausing (4 replicates) and diapausing mites (4 replicates) were used to generate Cy3- and Cy5-labeled cRNA respectively, using the Agilent Low Input Quick Amplification labeling kit (version 6.5, Agilent Technologies).
A total of 100 to 125 ng RNA was amplified and labeled with cy3-CTP following the Agilent Low Input Quick Amplification Labeling Kit protocol for One-Color Microarray-Based Gene Expression Analysis.
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RNA labeling (Quick RNA amplification and labeling kit; Agilent) and microarray hybridization (16 h at 60°C) were carried out according to the supplied manual.
In this work, it has been demonstrated that the use of NALFIA strips in combination with a quick PCR amplification step comprises most of the requirements for alternative rapid methods [ 6]: accurate, with very good sensitivity and specificity, reliable, and with a detection limit that enables the correct discrimination of the different E. coli virulence factors.
Database search and functional module assays explain in part why CB-EPCs amplification quicker.
Load capacity predictions provided by those analysis methods were used to develop regression models for a quick estimation of dynamic amplification factor at a given displacement/drift level.
Those experiments may include simple immunoprecipitations, a quick round of DNA amplifications, or simple restriction digests.
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