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Antibodies The referee questions specificity of the antibodies that we used: GFP-specific rabbit polyclonal IgG (ab290) from Abcam; calmyrin-specific rabbit polyclonal IgG (11823-1-AP) from ProteinTech Group, Inc. and CD34-specific monoclonal IgG 2a (sc-18917) from Santa Cruz.
The primary outcome of interest was the change in the proportion of reformulated questions that had each of the four dimensions of questions specificity explicitly described.
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We defined a properly-specified question as a question having all four dimensions of question specificity present.
‡ A properly specified question was one that had an explicit statement for each of the four dimensions of question specificity.
All requests submitted by eligible participants to the Evidence Centre from July 1, 2000 were prospectively analysed for completeness of each of the four basic dimensions of question specificity.
At baseline, approximately 20% of initially submitted questions in each of the groups was properly specified (an explicit statement describing each of the four dimensions of question specificity was present).
Previous research has explored patient's perception of the OHS, and found that patients encountered several limitations of the OHS relating to question specificity, response category clarity, exclusion of comorbidities, and experience of pain [ 23].
Our previous observation of H3K14 acetylation variation between two natural strains [ 6, 15] raised questions regarding specificity: Is this variation caused by one strain having a specific H3K14ac profile?
Therefore, another distinction operating in the representational criterion pertains to questions of specificity versus variety when selecting and using model organisms.
In this study, we used Surface Plasmon Resonance technique to answer the questions of specificity and sensitivity of thrombin detection by the thrombin-binding aptamers HD1, NU172 and HD22.
Practice answering questions with specificity so you are ready to show domain mastery.
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