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It's not just a question of sampling the goods, however.
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When a model of network dynamics is required then there is also the vexed question of sample rate and sample time selection to be resolved.
Of course, there is an important question of sample bias in the data [12], [14] (see also the Materials and Methods section), though its extensive use and utility, even in areas where data are scarce [13], is testament to the fact that it can yield important information if treated carefully.
The issue of power naturally leads to the question of sample size.
Following the question of samples with low cellularity (particularly in the case of neoadjuvant treatment), some authors recommend that a biopsy should be always dedicated to molecular biology before treatment, or to replace the step of macrodissection by laser microdissection [ 48].
Other buffers may not be equally suited to Coulter counter measurements and dilution in saline or other conductive solutions will raise the question of potential sample alterations caused by changes in solution conditions and the sample preparation process.
Although these debates are timely, they miss a crucial point at the beginning of the pipeline: the question of whether sample donors are able to access the raw data derived directly from their stored sample.
The Joint Research Centre, Petten, The Netherlands supported ENIQ by investigating the question of the sample size required to determine a reliable POD curve.
The nature of the spiked compounds should be related to the analytical question of the sample set.
The question of representative sample size in different studies is unclear and is dependent on study design [ 33].
However, the specific question of between-sample normalization is usually covered by measuring one or a few supposedly constant endogenous control genes.
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