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The percentage of expressing cells was calculated using the cell quest program.
Iwa is an Atlantic Bottlenose dolphin and part of the Dolphin Quest program.
Take a dip in one of its three swimming pools or four-acre saltwater lagoon or take part in its "dolphin quest" program, the only interactive dolphin program on the island that allows guests to touch, feed, and swim with these impressive creatures.
Analysis was done with the Cell Quest program.
The extent of apoptosis was determined by plotting PI fluorescence versus the forward scatter parameter, using the Cell Quest program.
Data acquisition was performed with the Cell Quest program (Becton-Dickinson) and analyzed with programs Paint-a-Gate and ModFit for the cell cycle profile (Becton-Dickinson).
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Maths quest programs allow children to learn in a gaming environment and have to go through various stages to complete a maths task.
Cells were washed and analyzed on a FACScan flow cytometer (Beckton Dickinson Immunocytometry System, San Jose, CA) with the Cell Quest software program.
Cells were washed and stained with FITC- anti-mouse IFN-γ, PE-anti-mouse IFN-γ, PE anti-mouse IL-10 or isotype controls (BD Biosciences Pharmingen) in FACS buffer with 0.5% saponin for 45 min at R.T. Cells were then washed in FACS buffer with 0.1% saponin and subsequently with FACS buffer with 0.01% saponin and then analyzed on a FACScan flow cytometer with the Cell Quest software program.
Primers and probes were designed using the online PrimerQuest® program (Integrated DNA Technologies, Coralville, IA) [ 15].
Data were collected from three different experiments and analyzed by BD Cell quest computer program.
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